Status
The Porphyridium purpureum CCMP1328 genome sequence and gene models were not determined by the Joint Genome Institute (JGI), but were downloaded from NCBI on April 29, 2022. In order to ensure this genome is comparable to those sequenced by the JGI, we applied filters to remove if present: 1) transposable elements, 2) pseudogenes, 3) alternative transcripts and overlapping models, 4) alleles on secondary scaffolds and 5) unsupported short models. This resulted in the removal of 1620 models from P. purpureum and the generation of the FilteredModels1 (GeneCatalog) gene track. All published models are available in the ExternalModels track. Please note that this copy of the genome is not maintained by NCBI and is therefore not automatically updated. In order to allow comparative analyses with other algal genomes sequenced by the JGI, a copy of this genome is incorporated into PhycoCosm. The JGI Annotation Pipeline was used to add functional annotation to this genome.
Porphyridium purpureum CCMP1328 is likely a diploid genome, and this is reflected in an assembly and annotation with significant separation of alleles. Many of the smaller scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertoire of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.
Genome Assembly | |
Genome Assembly size (Mbp) | 22.19 |
Sequencing read coverage depth | |
# of contigs | 52 |
# of scaffolds | 52 |
# of scaffolds >= 2Kbp | 52 |
Scaffold N50 | 4 |
Scaffold L50 (Mbp) | 1.85 |
# of gaps | 0 |
% of scaffold length in gaps | 0.0% |
Three largest Scaffolds (Mbp) | 5.72, 2.41, 2.31 |
Gene Models | FilteredModels1 | |
length (bp) of: | average | median |
gene | 1572 | 1293 |
transcript | 1545 | 1266 |
exon | 1359 | 1118 |
intron | 203 | 152 |
description: | ||
protein length (aa) | 515 | 422 |
exons per gene | 1.14 | 1 |
# of gene models | 8278 |
Genome Reference(s)
Bhattacharya D, Price DC, Chan CX, Qiu H, Rose N, Ball S, Weber AP, Arias MC, Henrissat B, Coutinho PM, Krishnan A, Zäuner S, Morath S, Hilliou F, Egizi A, Perrineau MM, Yoon HS
Genome of the red alga Porphyridium purpureum.
Nat Commun. 2013;4():1941. doi: 10.1038/ncomms2931
Lee J, Kim D, Bhattacharya D, Yoon HS
Expansion of phycobilisome linker gene families in mesophilic red algae.
Nat Commun. 2019 Oct 23;10(1):4823. doi: 10.1038/s41467-019-12779-1
Links
- JGI Algae EcoGroup Portal
- JGI PhyloGroup Portals: Rhodophyta Bangiophyceae