Info • Haematococcus lacustris Liv1 v1.2

Status

[October 2021] The genome was sequenced with PacBio and Illumina, assembled with MECAT, polished with ARROW, and annotated with the JGI Annotation Pipeline. The transcriptome consisted of 2 parts: 1) sequenced with Illumina and assembled with Trinity; 2) sequenced with PacBio and clustered into consensi with IsoSeq.

The genome is likely a polymorphic diploid, and this is reflected in an assembly and annotation with significant separation of alleles. Many of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertiore of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Genome Assembly
Genome Assembly size (Mbp) 291.51
Sequencing read coverage depth 297.30x
# of contigs 1648
# of scaffolds 1648
# of scaffolds >= 2Kbp 1648
Scaffold N50 222
Scaffold L50 (Mbp) 0.36
# of gaps 0
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 2.27, 1.87, 1.77


ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 105378 88202 83.7%
Ests est.fasta 430348284 225194898 52.3%


Gene Models FilteredModels1
length (bp) of: average median
gene 4319 3089
transcript 1673 1401
exon 234 132
intron 433 285
description:
protein length (aa) 422 317
exons per gene 7.14 6
# of gene models 21373


Links

Funding

The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.