Info • Entomoneis sp. UTEX 11IV18-2A FL v1.0

Status

[March 2023] The Entomoneis sp. UTEX 11IV18-2A FL v1.0 genome was sequenced with PacBio, assembled with HiFiAsm, and annotated with the JGI Annotation Pipeline. The transcriptome was sequenced with Illumina and assembled with Trinity.

This genome is likely a polymorphic diploid, and this is reflected in an assembly and annotation with significant separation of alleles. Some of the scaffolds are very similar to larger scaffolds and are predicted to constitute an alternate or secondary haplotype. To represent these primary and secondary haplotypes in the Portal, we have created 'primary alleles' and 'secondary alleles' gene model tracks, comprising the models found on each haplotype. The goal of the GeneCatalog (GC) is to produce a non-redundant set of models which captures the full functional repertoire of the genome, and so the few secondary alleles that are unique were included in the GC, while all others were not.

Genome Assembly
Genome Assembly size (Mbp) 459.75
Sequencing read coverage depth 58.26x
# of contigs 785
# of scaffolds 782
# of scaffolds >= 2Kbp 782
Scaffold N50 113
Scaffold L50 (Mbp) 1.17
# of gaps 3
% of scaffold length in gaps 0.0%
Three largest Scaffolds (Mbp) 5.26, 4.99, 4.33


ESTs Data set # sequences total # mapped to genome % mapped to genome
EstClusters ESTclusters 115808 59391 51.3%
Ests est.fasta 326123381 313066447 96.0%

  • Please note: Using BLAST, unmapped RNA contigs were found to hit bacteria, Acanthamoeba castellanii (amoeba), and Salmo trutta (fish), suggesting that the RNA library was contaminated.

Gene Models FilteredModels1
length (bp) of: average median
gene 1775 1330
transcript 1456 1150
exon 673 377
intron 277 92
description:
protein length (aa) 384 299
exons per gene 2.16 2
# of gene models 32720


Collaborators

Funding

The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.