Status
[May 2022] The genome and transcriptome sequences of Desmodesmus armatus SE107 were not determined by the Joint Genome Institute (JGI). The genome was sequenced with Pacbio and assembled with HGAP at the National Renewable Energy Lab (NREL). The transcriptome was provided by Sapphire Energy, and then assembled with Trinity by JGI. Subsequently, the JGI Annotation Pipeline was used to generate structural and functional annotations.
The genome assembly work was funded by the U.S. Department of Energy Office of Energy Efficiency and Renewable Energy and by the Rewiring Algal Carbon Energetics for Renewables (RACER) consortium, and by the National Renewable Energy Laboratory Research Data Initiative.
Genome Assembly | |
Genome Assembly size (Mbp) | 116.30 |
Sequencing read coverage depth | 85.0x |
# of contigs | 905 |
# of scaffolds | 905 |
# of scaffolds >= 2Kbp | 901 |
Scaffold N50 | 101 |
Scaffold L50 (Mbp) | 0.34 |
# of gaps | 0 |
% of scaffold length in gaps | 0.0% |
Three largest Scaffolds (Mbp) | 1.61, 1.61, 1.48 |
ESTs | Data set | # sequences total | # mapped to genome | % mapped to genome |
Ests | est.fasta | 319251230 | 293454463 | 91.9% |
Other | Sapphire_RNA_contigs | 196426 | 110487 | 56.2% |
- Please note: Using BLAST, unmapped RNA contigs were found to hit Brachionus plicatilis, Bos taurus, and Homo sapiens suggesting that the RNA library was contaminated.
Gene Models | FilteredModels1 | |
length (bp) of: | average | median |
gene | 3939 | 3061 |
transcript | 1762 | 1446 |
exon | 274 | 153 |
intron | 404 | 311 |
description: | ||
protein length (aa) | 417 | 326 |
exons per gene | 6.42 | 5 |
# of gene models | 17675 |
Collaborators
- Lieve Laurens at the National Renewable Energy Laboratory
Genome Reference(s)
Please cite the following publication(s) if you use the data from this genome in your research:
Knoshaug Eric P., Nag Ambarish, Astling David P., Douchi Damien, Laurens Lieve M. L.
Draft Genome Sequence of the Biofuel-Relevant Microalga Desmodesmus armatus
Microbiol Resour Announc. 2020;9(6): doi: 10.1128/mra.00896-19
Knoshaug Eric P., Nag Ambarish, Astling David P., Douchi Damien, Laurens Lieve M. L.
Draft Genome Sequence of the Biofuel-Relevant Microalga Desmodesmus armatus
Microbiol Resour Announc. 2020;9(6): doi: 10.1128/mra.00896-19
Links
- JGI Algae EcoGroup Portal
- JGI PhyloGroup Portals: Viridiplantae Chlorophyta Chlorophyceae Sphaeropleales Scenedesmaceae
Funding
The work conducted by the U.S. Department of Energy Joint Genome
Institute, a DOE Office of Science User Facility, is supported by
the Office of Science of the U.S. Department of Energy under
Contract No. DE-AC02-05CH11231.